Inhibition of prolyl endopeptidase by synthetic peptide fragments of human .BETA.-casein.
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چکیده
منابع مشابه
Slow tight-binding inhibition of prolyl endopeptidase by benzyloxycarbonyl-prolyl-prolinal.
Prolyl endopeptidase is a serine proteinase that specifically cleaves peptides on the carboxy side of proline residues. Wilk & Orlowski [(1983) J. Neurochem. 41, 69-75] have shown that benzyloxycarbonyl-prolyl-prolinal (Z-prolyl-prolinal) is a potent inhibitor of prolyl endopeptidase. We show that Z-prolyl-prolinal is a slow-binding inhibitor of mouse brain prolyl endopeptidase with Ki 0.35 +/-...
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Procollagen C-endopeptidase (BMP-1) and N-endopeptidase (ADAMTS-2) are key enzymes for correct and efficient conversion of fibrillar procollagens to their self assembling monomers. Thus, they have an essential role in building and controlling the quality of extracellular matrices (ECMs). Here, we tested inhibition of activity of the largest variant of BMP-1, a recombinant mammalian tolloid (mTl...
متن کاملPurification and characterization of human brain prolyl endopeptidase.
Prolyl endopeptidase (EC 3.4.21.26) was purified from human brain by a series of column-chromatographic steps using DEAE-cellulose DE-52, hydroxyapatite, phenyl-Sepharose, Sephacryl S-200 and f.p.l.c. (Mono Q). The enzyme was purified by a factor of 943 and was homogeneous in a SDS/polyacrylamide gel as judged by Coomassie Blue staining. The Mr estimated by SDS/PAGE is 79,600, and under native ...
متن کاملRegulation of prolyl endopeptidase activity by the intracellular redox state.
The activity of prolyl endopeptidase was markedly decreased during incubation of intact murine erythroleukemia cells at 45 degrees C, but not during incubation of sonicated cells or during incubation at 42 degrees C. The thermal inactivation of prolyl endopeptidase in situ required neither the synthesis of proteins and polynucleotides nor the synergistic activation of inhibitors. Moreover, inhi...
متن کاملStaurosporine, a prolyl endopeptidase inhibitor.
anti-umnesic efTect on Alzheimer's rats.fi) In PCI2・ h cells staurosporine blocked NGF-induced neuriLe outgrowth at low doses. but at hLgher doses PCI2h cclls responded te staurospovine blr' rupid generation of neurites.4) The meehanisms of neurite outgrewth prompted by staurosporine might be difTl:Fent from that ofNGF. dB-cAMP, or infection witli a retrovirus carr},ing the v-src oncogene.4] It...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1991
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.55.825